Spike Sorting
System
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The figure at left shows the proper
interconnections for discrimination and visual on-line inspection of extracellular
unit records. The DIS-1 multiplexed output is viewed on one trace of an
oscilloscope triggered by the threshold trigger output of the DIS-1. Small
bars in the drawing appear much as shown, indicating the precise upper and
lower bounds as well as the adjustable time of the discrimination, set by
the user to coincide with the portion of the wave-form most distinctive
from others exceeding the trigger level. To be certain that only one unit
has passed through the window and that others do not exist with waveforms
differing before the trigger, trigger an oscilloscope trace (storage mode
is particularly useful) from the acceptance pulse and look at a delayed
version of the input to the discriminator which has passed through an Analog
Delay (AD-3 and AD-6 dual delays now available). D1S-1's can be easily chained
for forward or backward multi-point discrimination. |
Other forms of data reduction can greatly
enhance on-line appreciation of the course of the experiment and reduce
or even eliminate the need for complex computer data processing or taping.
The BAK 1S1-1 Interspike Interval Converter generates a dot or level display
of the instantaneous frequency of firing (linear) of an accepted unit.
The BAK DTC-1 Digital Timer Counter can accurately track both total events
and latency from trigger events such as stimuli, with LED display and
BCD outputs. The BAK RG-1 Raster Stepper produces direct dot rastering
and chart recorder rate profiles plus analog contour displays. The DD-1
Digital Delay can let you look backwards at spike events preceding behavioral
criteria as shown.
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